Based on your description, the stain did not make you
niddah, because it was found on
colored underwear.
We usually advise that women with non-
niddah stains such as yours
abstain from relations for a day, as a precaution against a flow beginning during relations. Please note that this is a recommendation and not a strict halachic requirement. You can make a decision taking your personal considerations (your vacation, whether you are confident a flow will not begin) into account. Make sure to follow the
usual precautions against becoming
niddah unnecessarily from staining.
Please write back with any further questions.